Name: Eppig/Hampl oocyte Library ID: 1182 Organism: Mus musculus Strain: C57BL/6 Gender: female Age: 0 Stage: adult Organ: oocyte Tissue: oocyte isolated 46 hrs after PMSG injection Host: DH10B Vector: pSPORT1 Vector type: phagemid Insert digest: 5' SalI/NotI 3' Stop Codon Status: without Description: Cloned unidirectionally from mRNA prepared from oocytes isolated 46 hours after PMSG injection. NotI(dT) primer: 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'. SalI linker: 5'-TCGACCCACGCGTCCG-3'. cDNAs were size-selected for 500 bp and cloned into the SalI/NotI sites of pSPORT vector (Life Technologies). Average insert size: 1.5 kb. For aliquots of this library, please contact Dr. John Eppig, The Jackson Laboratory, 600 Main St., Bar Harbor ME 04609.