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cDNA library: Eppig/Hampl oocyte

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Information for 1 library


   1. Eppig/Hampl oocyte

Eppig/Hampl oocyte

Name: Eppig/Hampl oocyte
Library ID: 1182
Organism: Mus musculus
Strain: C57BL/6
Gender: female
Age: 0
Stage: adult
Organ: oocyte
Tissue: oocyte isolated 46 hrs after PMSG injection
Host: DH10B
Vector: pSPORT1
Vector type: phagemid
Insert digest: 5' SalI/NotI 3'
Stop Codon Status: without
Description: Cloned unidirectionally from mRNA prepared from oocytes isolated 46 hours after PMSG injection. NotI(dT) primer: 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'. SalI linker: 5'-TCGACCCACGCGTCCG-3'. cDNAs were size-selected for 500 bp and cloned into the SalI/NotI sites of pSPORT vector (Life Technologies). Average insert size: 1.5 kb. For aliquots of this library, please contact Dr. John Eppig, The Jackson Laboratory, 600 Main St., Bar Harbor ME 04609.

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