Name: UI-R-A1 Library ID: 1210 Organism: Rattus norvegicus Strain: Sprague-Dawley Age: 0 Organ: mixed Tissue: pool: ovary, lung (adult), placenta, brain, liver, kidney, spleen, heart, muscle Host: DH10B Vector: pT7T3D-PacI Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: This library is a subtracted library derived from the UI-R-A0 normalized pooled library, consisting of a mixture of individually tagged normalized libraries constructed from rat placenta (tag ATGTG), adult lung (TTCCA), brain (TAGAG), liver (CACAC), kidney (CAAAC), heart (ACAAC), spleen (GAGA), ovary (TCAC), and muscle (AAG). The tag is a string of 3-5 nucleotides present between the NotI site and the oligo-dT track which allow identification of the library of origin of a clone within the mixture. The UI-R-A1 subtracted library was constructed as follows: PCR amplified cDNA inserts from a pool of approximately 3840 UI-R-A0 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the UI-R-A0 library in the form of single-stranded circles. This procedure has been previously described in Genome Research 6:791-806. This library was constructed in the laboratory of M. Bento Soares, PhD. as part of the University of Iowa Rat EST Project.