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cDNA library: UI-R-E1

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Information for 1 library


   1. UI-R-E1


Name: UI-R-E1
Library ID: 1213
Organism: Rattus norvegicus
Strain: Sprague-Dawley
Age: 0
Stage: embryo
Organ: embryo
Tissue: pool (ages 8, 12 and 18 days postconception)
Host: DH10B
Vector: pT7T3D-PacI
Vector type: plasmid
Insert digest: 5' EcoRI/NotI 3'
Stop Codon Status: without
Description: This library is a subtracted library derived from the normalized pooled UI-R-E1 library (consisting of a mixture of individually tagged normalized libraries constructed from 8-day (tag AATG), 12-day (AATTG), and 18-day (AAAGG) embryos). The tag is a string of 3-5 nucleotides present between the NotI site and the oligo dT track which allows identification of the library of origin of a clone within the mixture. The UI-R-E1 subtracted library was constructed as follows: PCR amplified cDNA inserts from a pool of UI-R-E0 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the UI-R-E0 library in the form of single-stranded circles. This procedure has been previously described in Genome Research 6:791-806. This library was constructed in the laboratory of M. Bento Soares, PhD. as part of the University of Iowa Rat EST Project.

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