Name: UI-R-E1 Library ID: 1213 Organism: Rattus norvegicus Strain: Sprague-Dawley Age: 0 Stage: embryo Organ: embryo Tissue: pool (ages 8, 12 and 18 days postconception) Host: DH10B Vector: pT7T3D-PacI Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: This library is a subtracted library derived from the normalized pooled UI-R-E1 library (consisting of a mixture of individually tagged normalized libraries constructed from 8-day (tag AATG), 12-day (AATTG), and 18-day (AAAGG) embryos). The tag is a string of 3-5 nucleotides present between the NotI site and the oligo dT track which allows identification of the library of origin of a clone within the mixture. The UI-R-E1 subtracted library was constructed as follows: PCR amplified cDNA inserts from a pool of UI-R-E0 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the UI-R-E0 library in the form of single-stranded circles. This procedure has been previously described in Genome Research 6:791-806. This library was constructed in the laboratory of M. Bento Soares, PhD. as part of the University of Iowa Rat EST Project.
