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cDNA library: NCI_CGAP_Kid11

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Information for 1 library

kidney

   1. NCI_CGAP_Kid11

NCI_CGAP_Kid11

Name: NCI_CGAP_Kid11
Library ID: 1235
Organism: Homo sapiens
Age: 0
Stage: adult
Organ: kidney
Tissue: 2 pooled
Host: GeneHogs DH10B
Vector: pT7T3D-PacI
Vector type: plasmid
Insert digest: 5' EcoRI/NotI 3'
Stop Codon Status: without
Description: Plasmid DNA from the normalized library NCI_CGAP_Kid3 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1322376-1323911, 1456007-1456775, and 1500552-1502855). Subtraction by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.


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