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cDNA library: NCI_CGAP_Pr28

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Information for 1 library

prostate

   1. NCI_CGAP_Pr28

NCI_CGAP_Pr28

Name: NCI_CGAP_Pr28
Library ID: 1244
Organism: Homo sapiens
Gender: male
Age: 0
Stage: adult
Organ: prostate
Tissue: bulk tissue
Host: GeneHogs DH10B
Vector: pT7T3D-PacI
Vector type: plasmid
Insert digest: 5' EcoRI/NotI 3'
Stop Codon Status: without
Description: Plasmid DNA from the normalized library NCI_CGAP_Pr22 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 985608-986759, 1101192-1101959, and 1217928-1220615). Subtraction by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.

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