Name: Stanley Frontal B/N pool 1 Library ID: 1252 Organism: Homo sapiens Gender: both Age: 0 Stage: adult Organ: brain/CNS Tissue: frontal cortex pool: S-136 (35 yo female), S-65 (48 yo male), S-75 (bipolar 34 yo male), S-83 (biplolar 48 yo male) Host: GeneHogs DH10B Vector: pCR2.1-TOPO Vector type: plasmid Insert digest: EcoRI Stop Codon Status: without Description: Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of two male bipolars, ages 34 and 48 (S-75, S-83) subtracted by pool of two mentally normal individuals, male, age 48 and female, age 35 (S-65, S-136). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].
