Name: Stanley Frontal N/B pool 2 Library ID: 1256 Organism: Homo sapiens Gender: male Age: 0 Stage: adult Organ: brain/CNS Tissue: frontal cortex pool: S-111 (bipolar 45 yo male), S-124 (41 yo male), S-128 (bipolar 50 yo male), S-141 (53 yo male) Host: GeneHogs DH10B Vector: pCR2.1-TOPO Vector type: plasmid Insert digest: EcoRI Stop Codon Status: without Description: Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of two mentally normal males, ages 41 and 53 (S-124, S-141) subtracted by pool of two bipolar males, ages 45 and 50 (S-111, S-128). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, email@example.com].