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cDNA library: Stanley Hippocampus S/N pool 1

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Information for 1 library

brain/CNS

   1. Stanley Hippocampus S/N pool 1

Stanley Hippocampus S/N pool 1

Name: Stanley Hippocampus S/N pool 1
Library ID: 1257
Organism: Homo sapiens
Gender: both
Age: 0
Stage: adult
Organ: brain/CNS
Tissue: hippocampus pool: S-109 (schizophrenic 60 yo male), S-11 (schizophrenic 34 yo male), S-164 (50 yo female), S-64 (schizophrenic 58 yo male), S-65 (48 yo male), S-67 (34 yo male)
Host: GeneHogs DH10B
Vector: pCR2.1-TOPO
Vector type: plasmid
Insert digest: EcoRI
Stop Codon Status: without
Description: Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of three schizophrenic males, ages 34, 58 and 60 (S-11, S-64, S-109) subtracted by pool of three mentally normal individuals, male, ages 34 and 48, female, age 50 (S-65, S-67, S-164). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].

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