Name: Schiller glioblastoma multiforme Library ID: 1280 Organism: Homo sapiens Gender: female Age: 33 Stage: adult Organ: brain/CNS Tissue: glioblastoma multiforme, determined by consensus pathology. Tumor has LOH on chromosomes 10 and 14 and a gain in 9p and 1q. The tumor also has a heterozygous p53 mutation R273C. Host: SOLR Vector: pBluescript SK- Vector type: phagemid Insert digest: 5' EcoRI/XhoI 3' Stop Codon Status: without Description: Double-stranded cDNA was prepared from human glioblastoma multiforme usingprimer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. Tumor identification by consensus pathology; shows LOH on chromosomes 10 and 14 and a gain in 9p and 1q. The tumor also has a heterozygous p53 mutation R273C. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).