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cDNA library: Schiller glioblastoma multiforme

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Information for 1 library

brain/CNS

   1. Schiller glioblastoma multiforme

Schiller glioblastoma multiforme

Name: Schiller glioblastoma multiforme
Library ID: 1280
Organism: Homo sapiens
Gender: female
Age: 33
Stage: adult
Organ: brain/CNS
Tissue: glioblastoma multiforme, determined by consensus pathology. Tumor has LOH on chromosomes 10 and 14 and a gain in 9p and 1q. The tumor also has a heterozygous p53 mutation R273C.
Host: SOLR
Vector: pBluescript SK-
Vector type: phagemid
Insert digest: 5' EcoRI/XhoI 3'
Stop Codon Status: without
Description: Double-stranded cDNA was prepared from human glioblastoma multiforme usingprimer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. Tumor identification by consensus pathology; shows LOH on chromosomes 10 and 14 and a gain in 9p and 1q. The tumor also has a heterozygous p53 mutation R273C. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).

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