Name: Schiller AtT-20, RESP18 induced Library ID: 1282 Organism: Mus musculus Age: 0 Organ: pituitary gland Tissue: cell line, AtT-20, RESP18 induced Host: SOLR Vector: pBluescript SK- Vector type: phagemid Insert digest: 5' EcoRI/XhoI 3' Stop Codon Status: without Description: Double-stranded cDNA was prepared from cell line AtT-20 usingprimer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. This cell line was engineered for inducible overexpression of RESP18 using the rTET system. Cells were induced such that RESP18 protein would traverse the ER to the Golgi and induce the RESP18-activated signaling pathway. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).