Name: Schiller MAC13 Library ID: 1283 Organism: Mus musculus Age: 0 Organ: colon Tissue: colon cancer cell line MAC13 Host: SOLR Vector: pBluescript SK- Vector type: phagemid Insert digest: 5' EcoRI/XhoI 3' Stop Codon Status: without Description: Double-stranded cDNA was prepared from cell line MAC13 usingprimer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).