I.M.A.G.E. Consortium
“Sharing resources to achieve a common goal — the discovery of all genes”
cDNA library: Rashbass MOV-9.5
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Information for 1 library
eye
1.
Rashbass MOV-9.5
Rashbass MOV-9.5
Name:
Rashbass MOV-9.5
Library ID:
1290
Organism:
Mus musculus
Strain:
CD-1
Age:
0
Stage:
embryo
Organ:
eye
Tissue:
developing eye including optic vesicle and lens placode
Host:
DH10B
Vector:
pSPORT1
Vector type:
phagemid
Insert digest:
5' SalI/NotI 3'
Stop Codon Status:
without
Description:
mRNA made from developing eye tissue, cDNA made by oligo-dT priming with NotI oligo. SalI adaptor (5'-TCGACCCACGCGTCCG-3') ligated to 5' ends. Size-selected with cDNA size fractionation resin, average insert size 1.3 kb. Primary library, non-amplified. Library constructed by Dr. Pen Rashbass (penr@hgu.mrc.ac.uk).
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