Name: NCI_CGAP_Kid12 Library ID: 1309 Organism: Homo sapiens Age: 0 Stage: adult Organ: kidney Tissue: 2 pooled tumors, clear cell type Host: GeneHogs DH10B Vector: pT7T3D-PacI Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: Plasmid DNA from the normalized library NCI_CGAP_Kid5 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1323912-1325831, 1471368-1472903 and 1492104-1493255). Subtraction by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.