Name: Barstead MPL-RB14 Library ID: 1314 Organism: Mus musculus Gender: male Age: 0 Organ: muscle Host: GeneHogs DH10B Vector: pT7T3D-PacI Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [5' AATTCGTCGACAAG 3' and 5' CTTGTCGACG 3'], digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library constructed by R. Barstead (Oklahoma Medical Research Foundation).
