Name: Sugano-Kawakami DRA Library ID: 1333 Organism: Danio rerio Strain: AB Gender: both Age: 0 Stage: adult Organ: whole body Tissue: whole body (including unfertilized eggs) Host: GeneHogs DH10B Vector: pME18S-FL3 Vector type: phagemid Insert digest: 5' DraIII/DraIII 3' Stop Codon Status: without Description: 1st strand cDNA was primed with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [TGTTGGCCTACTGG], digested and cloned into distinct DraIII sites of the pME18S-FL3 vector (5' site CACTGTGTG, 3' site CACCATGTG). XhoI should be used to isolate the cDNA insert. Size selection was performed to exclude fragments <1.5kb. Library constructed and donated by Dr. Sumio Sugano and Dr. Ko-ichi Kawakami (University of Tokyo Institute of Medical Science). Custom primers for sequencing: 5' end primer CTTCTGCTCTAAAAGCTGCG and 3' end primer CGACCTGCAGCTCGAGCACA. REFERENCES: Suzuki, Y., Yoshitomo, K., Maruyama, K., Suyama, A., and Sugano, S. Construction and characterization of a full length-enriched and a 5' end enriched cDNA library. Gene 200, 149-156, 1997.