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cDNA library: Soares/Dieckgraefe NHCD

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Information for 1 library

colon

   1. Soares/Dieckgraefe NHCD

Soares/Dieckgraefe NHCD

Name: Soares/Dieckgraefe NHCD
Library ID: 1336
Organism: Homo sapiens
Age: 0
Stage: adult
Organ: colon
Tissue: colonic mucosa isolated from patients with moderate to severe Crohn's disease; including perforating (fistulas)and non-perforating samples.
Host: GeneHogs DH10B
Vector: pT7T3D-PacI
Vector type: plasmid
Insert digest: 5' EcoRI/NotI 3'
Stop Codon Status: without
Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCGTCTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization. Tissue samples provided by Dr. Brian Dieckgraefe (Washington University, dieck@im.wustl.edu); colonic mucosa represents a range of disease involvement from moderate to severe Crohn's disease; samples include both perforating (fistulas) and non-perforating samples. Library constructed by Bento Soares and M. Fatima Bonaldo.

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