Name: Soares/Dieckgraefe NHCD Library ID: 1336 Organism: Homo sapiens Age: 0 Stage: adult Organ: colon Tissue: colonic mucosa isolated from patients with moderate to severe Crohn's disease; including perforating (fistulas)and non-perforating samples. Host: GeneHogs DH10B Vector: pT7T3D-PacI Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCGTCTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization. Tissue samples provided by Dr. Brian Dieckgraefe (Washington University, firstname.lastname@example.org); colonic mucosa represents a range of disease involvement from moderate to severe Crohn's disease; samples include both perforating (fistulas) and non-perforating samples. Library constructed by Bento Soares and M. Fatima Bonaldo.