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cDNA library: NCI_CGAP_Ov18

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Information for 1 library

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   1. NCI_CGAP_Ov18

NCI_CGAP_Ov18

Name: NCI_CGAP_Ov18
Library ID: 1345
Organism: Homo sapiens
Gender: female
Age: 0
Stage: adult
Organ: ovary
Tissue: ovary, fibrotheoma
Host: GeneHogs DH10B
Vector: pT7T3D-PacI
Vector type: plasmid
Insert digest: 5' EcoRI/NotI 3'
Stop Codon Status: without
Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGCGACATTTTTTTTTTTTTTTTTT 3']; double- stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.

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