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cDNA library: Soares NMIE

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Information for 1 library

inner ear

   1. Soares NMIE

Soares NMIE

Name: Soares NMIE
Library ID: 1397
Organism: Mus musculus
Strain: C3H x 101
Gender: male
Age: 0
Stage: infant
Organ: inner ear
Tissue: pool of 170
Host: GeneHogs DH10B
Vector: pT7T3D-PacI
Vector type: plasmid
Insert digest: 5' EcoRI/NotI 3'
Stop Codon Status: without
Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCACACTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3'(Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized, and was constructed and donated by Bento Soares and M.Fatima Bonaldo (University of Iowa) and R. Hardisty, A. Varela-Carver, P. Mburu and S.D.M. Brown (MRC UK Mouse Genome Centre and Mammalian Genetics Unit, Harwell, UK).

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