Name: NIH_MGC_20 Library ID: 1430 Organism: Homo sapiens Age: 0 Organ: skin Tissue: melanotic melanoma cell line Host: GeneHogs DH10B Vector: pOTB7 Vector type: plasmid Insert digest: 5' EcoRI/XhoI 3' Stop Codon Status: without Description: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library.