Name: UI-R-AF1 Library ID: 1488 Organism: Rattus norvegicus Strain: Sprague-Dawley Age: 0 Stage: embryo Organ: heart Tissue: atrioventricular canal Host: DH10B Vector: pT7T3D-PacI Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: This normalized library has the tag GAAGG. The tag is a string of 3-5 nucleotides present between the NotI site and the oligo dT track which allows identification of the library of origin of a clone. The library was constructed as described in Genome Research 6:791-806. Tissue provided by Jim Lin, Department of Biology, University of Iowa. This library was constructed in the laboratory of M. Bento Soares, PhD. as part of the University of Iowa Rat EST Project.