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cDNA library: UI-R-C1

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Information for 1 library

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   1. UI-R-C1

UI-R-C1

Name: UI-R-C1
Library ID: 1491
Organism: Rattus norvegicus
Strain: Sprague-Dawley
Age: 0
Organ: mixed
Tissue: embryo (8, 12 and 18 days postconception), ovary, lung, placenta, brain, liver, kidney, heart, spleen, muscle
Host: DH10B
Vector: pT7T3D-PacI
Vector type: plasmid
Insert digest: 5' EcoRI/NotI 3'
Stop Codon Status: without
Description: This library is a subtracted library derived from the UI-R-C0 subtracted library (consisting of a mixture of individually tagged normalized libraries constructed from rat placenta (tag ATGTG), adult lung (TTCCA), brain (TAGAG), liver (CACAC), kidney (CAAAC), heart (ACAAC), spleen (GAGA), ovary (TCAC), muscle (AAG), 8-day (tag AATG), 12-day (AATTG), and 18-day (AAAGG) embryos. The tag is a string of 3-5 nucleotides present between the NotI site and the oligo-dT track which allow identification of the library of origin of a clone within the mixture. The UI-R-C1 subtracted library was constructed as follows: PCR amplified cDNA inserts from a pool of UI-R-C0 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the UI-R-C0 library in the form of single-stranded circles. This procedure has been previously described in Genome Research 6:791-806. This library was constructed in the laboratory of M. Bento Soares, PhD. as part of the University of Iowa Rat EST Project.

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