Name: UI-R-Y0 Library ID: 1493 Organism: Rattus norvegicus Strain: Sprague-Dawley Age: 0 Organ: eye Tissue: whole eye minus the lens Host: DH10B Vector: pT7T3D-PacI Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: This library is a subtracted library derived frmo an individually-tagged normalized whole-eye (minus the lens) library. The driver for the subtraction consisted of a pool of previous libraries: UI-R-A0, UI-R-A1, UI-R-E0, UI-R-E1, UI-R-C0, and UI-R-C1. The UI-R-Y0 subtracted library was constructed as follows: PCR amplified cDNA inserts from previous library clones from which 3' ESTs had been derived was used as a driver in a hybridization with the normalized whole eye library in the form of single-stranded circles. This procedure has been previously described in Genome Research 6:791-806. This library was constructed in the laboratory of M. Bento Soares, PhD. as part of the University of Iowa Rat EST Project.