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cDNA library: UI-R-Y0

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Information for 1 library

eye

   1. UI-R-Y0

UI-R-Y0

Name: UI-R-Y0
Library ID: 1493
Organism: Rattus norvegicus
Strain: Sprague-Dawley
Age: 0
Organ: eye
Tissue: whole eye minus the lens
Host: DH10B
Vector: pT7T3D-PacI
Vector type: plasmid
Insert digest: 5' EcoRI/NotI 3'
Stop Codon Status: without
Description: This library is a subtracted library derived frmo an individually-tagged normalized whole-eye (minus the lens) library. The driver for the subtraction consisted of a pool of previous libraries: UI-R-A0, UI-R-A1, UI-R-E0, UI-R-E1, UI-R-C0, and UI-R-C1. The UI-R-Y0 subtracted library was constructed as follows: PCR amplified cDNA inserts from previous library clones from which 3' ESTs had been derived was used as a driver in a hybridization with the normalized whole eye library in the form of single-stranded circles. This procedure has been previously described in Genome Research 6:791-806. This library was constructed in the laboratory of M. Bento Soares, PhD. as part of the University of Iowa Rat EST Project.

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