Name: UI-R-C2p Library ID: 1495 Organism: Rattus norvegicus Strain: Sprague-Dawley Age: 0 Organ: mixed Tissue: embryo (8, 12 and 18 days postconception), ovary, lung, placenta, brain, liver, kidney, heart, spleen, muscle Host: DH10B Vector: pT7T3D-PacI Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: This library is a subtracted library derived from the UI-R-C1 subtracted library, derived from the UI-R-C0 library (consisting of a mixture of individually tagged normalized libraries constructed from rat placenta (tag ATGTG), adult lung (TTCCA), brain (TAGAG), liver (CACAC), kidney (CAAAC), heart (ACAAC), spleen (GAGA), ovary (TCAC), muscle (AAG), 8-day (tag AATG), 12-day (AATTG), and 18-day (AAAGG) embryos. The tag is a string of 3-5 nucleotides present between the NotI site and the oligo-dT track which allow identification of the library of origin of a clone within the mixture. The UI-R-C2p subtracted library was constructed as follows: PCR amplified cDNA inserts from a pool of UI-R-C1 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the UI-R-C1 library in the form of single-stranded circles. This procedure has been previously described in Genome Research 6:791-806. This library was constructed in the laboratory of M. Bento Soares, PhD. as part of the University of Iowa Rat EST Project.
