Name: NIH_MGC_50 Library ID: 1507 Organism: Homo sapiens Age: 0 Organ: lymph Tissue: germinal center B-cells mRNA size selected 3.5-4.4 kb Host: DH10B Vector: pT7T3D-PacI Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: Library was derived from 3.5-4.4 kb mRNA size fraction;1st strand cDNA was primed with a Not I - oligo(dT) primer: 5''-TGTTACCAATCTGAAGTGGGAGCGGCCGCATATGACTTTTTTTTTTTTTTTTTTTTTTTTT-3''; double-stranded cDNA was ligated to EcoRI adaptors 5''-AATTCGGCACGAGG-3'' and 5''-CCTCGTGCCG-3'' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library was constructed by Bento Soares and M.Fatima Bonaldo (University of Iowa). Note: this is a NIH_MGC Library.
