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cDNA library: NIH_MGC_50

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Information for 1 library

lymph

   1. NIH_MGC_50

NIH_MGC_50

Name: NIH_MGC_50
Library ID: 1507
Organism: Homo sapiens
Age: 0
Organ: lymph
Tissue: germinal center B-cells mRNA size selected 3.5-4.4 kb
Host: DH10B
Vector: pT7T3D-PacI
Vector type: plasmid
Insert digest: 5' EcoRI/NotI 3'
Stop Codon Status: without
Description: Library was derived from 3.5-4.4 kb mRNA size fraction;1st strand cDNA was primed with a Not I - oligo(dT) primer: 5''-TGTTACCAATCTGAAGTGGGAGCGGCCGCATATGACTTTTTTTTTTTTTTTTTTTTTTTTT-3''; double-stranded cDNA was ligated to EcoRI adaptors 5''-AATTCGGCACGAGG-3'' and 5''-CCTCGTGCCG-3'' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library was constructed by Bento Soares and M.Fatima Bonaldo (University of Iowa). Note: this is a NIH_MGC Library.

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