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cDNA library: NIH_MGC_62

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Information for 1 library

skin

   1. NIH_MGC_62

NIH_MGC_62

Name: NIH_MGC_62
Library ID: 1590
Organism: Homo sapiens
Age: 0
Organ: skin
Tissue: melanotic melanoma, high MDR, cell line
Host: GeneHogs DH10B
Vector: pDNR-LIB
Vector type: plasmid
Insert digest: SfiI (directional)
Stop Codon Status: without
Description: Double-stranded cDNA was prepared from cell line RNA. 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.75 kb (range 0.9-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library.

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