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cDNA library: X. laevis oocytes stage 5-6 mixed

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Information for 1 library

oocyte

   1. X. laevis oocytes stage 5-6 mixed

X. laevis oocytes stage 5-6 mixed

Name: X. laevis oocytes stage 5-6 mixed
Library ID: 1594
Organism: Xenopus laevis
Age: 0
Organ: oocyte
Host: TOP10
Vector: pBluescript SK-
Vector type: phagemid
Insert digest: 5' EcoRI/XhoI 3'
Stop Codon Status: without
Description: cDNA from 2 ug polyA+ mRNA, oligo dT-primed using 5'-ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTT-3'. Library was mass excised (from lambda-ZAPII) using ExAssist phage. The resulting single-stranded phagemids were used to infect TOP10F'. This library has been used successfully to clone a number of full-length cDNAs ranging in size from 1.4-4.5 kb. There are <0.5% clones with multiple inserts. One should be suspicious of any clone which gives 3 or more bands in an EcoRI/XhoI digest AND has an internal XhoI site. Library constructed and donated by B. Blumberg (University of California, Irvine); also referred to as Xenopus_laevis_ooctye_non_normalized.

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