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cDNA library: McCarrey/Eddy type A spermatogonia

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Information for 1 library


   1. McCarrey/Eddy type A spermatogonia

McCarrey/Eddy type A spermatogonia

Name: McCarrey/Eddy type A spermatogonia
Library ID: 1595
Organism: Mus musculus
Strain: CD-1
Gender: male
Age: 0
Stage: juvenile
Organ: testis
Tissue: type A spermatogonia, pooled from multiple mice
Host: GeneHogs DH10B
Vector: pBluescript SK+
Vector type: phagemid
Insert digest: 5' EcoRI/XhoI 3'
Stop Codon Status: without
Description: cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of 400bp material gives average insert size ranging from 1-2 kb. Library was mass excised (from lambda-UniZAP-XR) and resulting single-stranded phagemids were prepped and tranformed into DH10B. Library contains 96.5% recombinants. References: J. Androl. 20:635-639 and Gene 25:263-269. Library constructed and donated by J. McCarrey, Ph.D. (Southwest Foundation for Biomedical Research, Dept. of Genetics); excision done by E.M. Eddy, Ph.D. (National Institutes of Health, National Institute of Environmental Health Sciences). Original lambda-based library is available through ATCC, catalog #63416.

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