Name: McCarrey/Eddy spermatocytes Library ID: 1597 Organism: Mus musculus Strain: CD-1 Gender: male Age: 0 Stage: adult Organ: testis Tissue: pachytene spermatocytes, pooled from multiple mice Host: GeneHogs DH10B Vector: pBluescript SK+ Vector type: phagemid Insert digest: 5' EcoRI/XhoI 3' Stop Codon Status: without Description: cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of 400bp material gives average insert size ranging from 1-2 kb. Library was mass excised (from lambda-UniZAP-XR) and resulting single-stranded phagemids were prepped and tranformed into DH10B. Library contains 98% recombinants. References: J. Androl. 20:635-639 and Gene 25:263-269. Library constructed and donated by J. McCarrey, Ph.D. (Southwest Foundation for Biomedical Research, Dept. of Genetics); excision done by E.M. Eddy, Ph.D. (National Institutes of Health, National Institute of Environmental Health Sciences). Original lambda-based library is available through ATCC, catalog #63422.