Biosciences Directorate logo

I.M.A.G.E. Consortium

“Sharing resources to achieve a common goal — the discovery of all genes”

blue/green horizontal line

cDNA library: McCarrey/Eddy 18-day preleptotene spermatocytes

Home | Site Map | I.Q. search | Libraries by Species | Clones | Vectors | Primers | QC | Arrayed Clones by: Library | Tissue |

Information for 1 library

testis

   1. McCarrey/Eddy 18-day preleptotene spermatocytes

McCarrey/Eddy 18-day preleptotene spermatocytes

Name: McCarrey/Eddy 18-day preleptotene spermatocytes
Library ID: 1634
Organism: Mus musculus
Gender: male
Age: 0
Organ: testis
Tissue: 18-day preleptotene spermatocytes
Host: GeneHogs DH10B
Vector: pBluescript SK+
Vector type: phagemid
Insert digest: 5' EcoRI/XhoI 3'
Stop Codon Status: without
Description: cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of 400bp material gives average insert size ranging from 1-2 kb. Library was mass excised (from lambda-UniZAP-XR) and resulting single-stranded phagemids were prepped and tranformed into DH10B. Library constructed and donated by J. McCarrey, Ph.D. (Southwest Foundation for Biomedical Research, Dept. of Genetics); excision done by E.M. Eddy, Ph.D. (National Institutes of Health, National Institute of Environmental Health Sciences).

horizontal blue line

Home | I.M.A.G.E. | ORFeome | CGAP | MGC | XGC | ZGC | CMLS | Lawrence Livermore | U Iowa | LLNL Disclaimer | HAIB Disclaimer
Web page maintained by
preston.hunter@asu.edu
Email address:
Biological Questions and Comments to
preston.hunter@asu.edu