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cDNA library: McCarrey/Eddy 18-day preleptotene spermatocytes

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Information for 1 library


   1. McCarrey/Eddy 18-day preleptotene spermatocytes

McCarrey/Eddy 18-day preleptotene spermatocytes

Name: McCarrey/Eddy 18-day preleptotene spermatocytes
Library ID: 1634
Organism: Mus musculus
Gender: male
Age: 0
Organ: testis
Tissue: 18-day preleptotene spermatocytes
Host: GeneHogs DH10B
Vector: pBluescript SK+
Vector type: phagemid
Insert digest: 5' EcoRI/XhoI 3'
Stop Codon Status: without
Description: cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of 400bp material gives average insert size ranging from 1-2 kb. Library was mass excised (from lambda-UniZAP-XR) and resulting single-stranded phagemids were prepped and tranformed into DH10B. Library constructed and donated by J. McCarrey, Ph.D. (Southwest Foundation for Biomedical Research, Dept. of Genetics); excision done by E.M. Eddy, Ph.D. (National Institutes of Health, National Institute of Environmental Health Sciences).

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