Name: McCarrey/Eddy 18-day leptotene and zygotene spermatocytes Library ID: 1635 Organism: Mus musculus Gender: male Age: 0 Organ: testis Tissue: 18-day leptotene and zygotene spermatocytes Host: GeneHogs DH10B Vector: pBluescript SK+ Vector type: phagemid Insert digest: 5' EcoRI/XhoI 3' Stop Codon Status: without Description: cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of 400bp material gives average insert size ranging from 1-2 kb. Library was mass excised (from lambda-UniZAP-XR) and resulting single-stranded phagemids were prepped and tranformed into DH10B. Library constructed and donated by J. McCarrey, Ph.D. (Southwest Foundation for Biomedical Research, Dept. of Genetics); excision done by E.M. Eddy, Ph.D. (National Institutes of Health, National Institute of Environmental Health Sciences).
