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cDNA library: Soares NMTC

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Information for 1 library

trophoblast

   1. Soares NMTC

Soares NMTC

Name: Soares NMTC
Library ID: 1681
Organism: Mus musculus
Age: 0
Organ: trophoblast
Tissue: trophoblast cells
Host: GeneHogs DH10B
Vector: pT7T3D-PacI
Vector type: plasmid
Insert digest: 5' EcoRI/NotI 3'
Stop Codon Status: without
Description: 1st strand cDNA was primed with a NotI - oligo(dT) primer 5'-AACTGGAAGAATTCGCGGCCGCGGTTGTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed in the laboratory of M. Bento Soares (University of Iowa).

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