Name: Soares NMBP2 Library ID: 1682 Organism: Mus musculus Age: 0 Stage: embryo Organ: brain/CNS Tissue: pituitary gland Host: GeneHogs DH10B Vector: pT7T3D-PacI Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: 1st strand cDNA was primed with a NotI- oligo(dT) primer 5'-AACTGGAAGAATTCGCGGCCGCGGCGCTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3'(Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacII vector. Library went through one round of normalization, and was constructed in the laboratory of M. Bento Soares (University of Iowa).
