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cDNA library: Soares NMO1

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Information for 1 library

ovary

   1. Soares NMO1

Soares NMO1

Name: Soares NMO1
Library ID: 1684
Organism: Mus musculus
Gender: female
Age: 0
Organ: ovary
Tissue: 6 and 10 hours post-treatment with PMSG/hCG
Host: GeneHogs DH10B
Vector: pT7T3D-PacI
Vector type: plasmid
Insert digest: 5' EcoRI/NotI 3'
Stop Codon Status: without
Description: 1st strand cDNA was primed with a NotI - oligo(dT) primer 5'-AACTGGAAGAATTCGCGGCCGCAACAGTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed in the laboratory of M. Bento Soares (University of Iowa).

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