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cDNA library: Zebrafish SJD adult male

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Information for 1 library

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   1. Zebrafish SJD adult male

Zebrafish SJD adult male

Name: Zebrafish SJD adult male
Library ID: 1763
Organism: Danio rerio
Gender: male
Age: 0
Stage: adult
Organ: whole body
Host: GeneHogs DH10B
Vector: pZERO-2
Vector type: plasmid
Insert digest: 5' EcoRI/XhoI 3'
Stop Codon Status: without
Description: First strand cDNA was primed using an oligo-dT adaptor primer with an XhoI site: 5'-gagagagagagagagagagaactagtctcgagtttttttttttttttttt-3'. Double-stranded cDNA was ligated to an EcoRI adaptor: 5'-aattcggcacgagg-3' and was digested with XhoI and directionally cloned into pZero-2. Library construction by Joe Barnes and Steve Johnson (Washington University).

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