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cDNA library: Gong zebrafish testis

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Information for 1 library

testis

   1. Gong zebrafish testis

Gong zebrafish testis

Name: Gong zebrafish testis
Library ID: 1776
Organism: Danio rerio
Gender: male
Age: 0
Stage: adult
Organ: testis
Tissue: pooled testes (from 31 fish)
Host: DH10B TonA
Vector: pBluescript SK-
Vector type: phagemid
Insert digest: 5' EcoRI/XhoI 3'
Stop Codon Status: without
Description: Poly A+ RNA was isolatd from the testes of 31 male adult zebrafish (4-5 month old). cDNAs were made using oligo-dT primers and inserted into lambda ZAP II vector (Stratagene) by Dr. Z. Gong, in vivo mass-excised to pBluescript SK- following the Washington University protocol (http://genome.wustl.edu/est/lambda_protocol.shtml). Please contact Zhiyuan Gong for further information on this library (National University of Singapore, Department of Biological Sciences, Lower Kent Ridge Road, Singapore 119260).

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