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cDNA library: Zebrafish SJD day 3 embryo

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Information for 1 library

embryo

   1. Zebrafish SJD day 3 embryo

Zebrafish SJD day 3 embryo

Name: Zebrafish SJD day 3 embryo
Library ID: 1853
Organism: Danio rerio
Age: 0
Stage: embryo
Organ: embryo
Host: DH10B
Vector: pAMP1
Vector type: phagemid
Insert digest: 5' NotI/EcoRI 3'
Stop Codon Status: without
Description: First strand cDNA synthesis was primed using oligo-dT on magnetic beads with an additional primer 5'-gcggccgctaatacgactcacta-taggg-3'. Second strand synthesis was a 3-cycle PCR using the primers 5'-ggccgctaatacgactcactatag-3' and 5'aagcagtggtaacaacgcagagtactttttttttttttttvn-3'. cDNA was subsequently amplified in a 7-cycle PCR with the following primers: 5'-ggccgctaatacgactcactatag-3' and 5'-aagcagtggt-aacaacgcag. Deoxy-UMP adaptors were added in a third PCR (5 cycles) and the primers 5'-caucaucaucauggccgctaatacgactcactataggg-3' and 5'-cuacuacuacuaaagcagtggtaacaacgcagagtac-3'. Ends were treated with uracil DNA glycosylase and product with 3' overhangs was annealed to complementary ends of pAMP1. Insert can be excised using EcoRI and NotI. Library

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