Name: Soares NMPR0 Library ID: 1879 Organism: Mus musculus Gender: male Age: 0 Stage: infant Organ: prostate Host: DH10B TonA Vector: pT7T3D-PacI Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCTATAGACGCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized. A non-normalized version of this library was also constructed, Soares MPR0. Library was constructed by Bento Soares and M.Fatima Bonaldo (University of Iowa).
