Name: NIH_MGC_128 Library ID: 1891 Organism: Homo sapiens Gender: both Age: 0 Stage: embryo Organ: brain/CNS Tissue: normal brain, pooled Host: DH10B TonA Vector: pDNR-LIB Vector type: plasmid Insert digest: SfiI (directional) Stop Codon Status: without Description: Double-stranded cDNA was prepared from a pool of 40 cell line polyA+ RNAs (bladder - 2%, blood - 33.4%, brain - 5.6%, breast - 12.5%, colon - 4%, connective tissue - 1.4%, eye - 1%, intestine - 2.6%, kidnney - 2.2%, liver - 5.7%, lung - 10.8%, NK-cell - 5.2%, ovary - 4%, pharynx - 2.5%, prostate - 4.3%, salivary gland - 1.3%, and skin - 2.3%). 5'' and 3'' adaptors were used in cloning as follows: 5''-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3'' and 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3''. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected to contain the >2 kb size fraction (other fractions present in NIH_MGC_126 and NIH_MGC_127). Library created in the laboratory of T. Usdin, M.D., Ph.D. (NIMH, NIH). Note: this is a NIH_MGC Library.
