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cDNA library: Amplified Melton mouse islets 1 MIS1-A

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Information for 1 library


   1. Amplified Melton mouse islets 1 MIS1-A

Amplified Melton mouse islets 1 MIS1-A

Name: Amplified Melton mouse islets 1 MIS1-A
Library ID: 1899
Organism: Mus musculus
Strain: ICR
Gender: male
Age: 0
Stage: adult
Organ: pancreas
Tissue: Islets of Langerhans, pooled
Host: DH10B
Vector: pSPORT1
Vector type: phagemid
Insert digest: 5' SalI/NotI 3'
Stop Codon Status: without
Description: Library constructed using SuperScript Plasmid Library kit (Life Technologies) and 5' linker sequences as follows: 5'-TCGACCCACGCGTCCG-3' and 3'-GGGTGCGCAGGC-5'. cDNA made by oligo-dT priming using 5'-PGACTAGTTCTAGATCGCGAGCGGCCGCCCT(15)-3'. Size-selected by column fractionation; average insert size 0.91 kb. Amplified once on solid support. cDNA Library Preparation: G. Chen in the laboratory of D. Melton, Ph.D. (Harvard University)

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