Name: Melton mouse adult pancreas 1 MAZ1 Library ID: 1901 Organism: Mus musculus Strain: ICR Gender: male Age: 0 Stage: adult Organ: pancreas Tissue: normal pancreas, pooled Host: TOP10 Vector: pZERO-2 Vector type: plasmid Insert digest: 5' XhoI/NotI 3' Stop Codon Status: without Description: Library constructed using SuperScript Plasmid Library kit (Life Technologies) and 5' linker sequences as follows: 5'-TCGACCCACGCGTCCG-3' and 3'-GGGTGCGCAGGC-5'. cDNA made by oligo-dT priming using 5'-PGACTAGTTCTAGATCGCGAGCGGCCGCCCT(15)-3'. XhoI site destroyed during cloning (excise insert using XbaI/NotI). Size-selected by column fractionation. Primary library, unamplified. Library constructed and arrayed in the laboratory of D. Melton, Ph.D. (Harvard University).