Name: Melton human islets HIZ1 Library ID: 1909 Organism: Homo sapiens Gender: both Age: 0 Stage: adult Organ: pancreas Tissue: Islets of Langerhans, pooled Host: TOP10 Vector: pZERO-2 Vector type: plasmid Insert digest: 5' XhoI/NotI 3' Stop Codon Status: without Description: Library constructed using SuperScript Plasmid Library kit (Life Technologies) and 5' linker sequences as follows. 5'-TCGACCCACGCGTCCG-3' and 3'-GGGTGCGCAGGC-5'. cDNA made by oligo-dT priming using 5'-PGACTAGTTCTAGATCGCGAGCGGCCGCCCT(15)-3'. XhoI site destroyed during cloning (excise insert using XbaI/NotI). Size-selected by column fractionation; average insert size 1.59kb. Primary library, unamplified. Library constructed and arrayed in the laboratory of D. Melton, Ph.D. (Harvard University).