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cDNA library: NCI_CGAP_Fs1

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Information for 1 library

bone

   1. NCI_CGAP_Fs1

NCI_CGAP_Fs1

Name: NCI_CGAP_Fs1
Library ID: 1924
Organism: Homo sapiens
Age: 0
Organ: bone
Tissue: fibrosarcoma, cell line HT-1088 (ATCC #CCL-121)
Host: DH10B
Vector: pT7T3D-PacI
Vector type: plasmid
Insert digest: 5' EcoRI/NotI 3'
Stop Codon Status: without
Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGTTCTACGAGTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.

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