Name: Xenopus laevis oocyte Library ID: 1931 Organism: Xenopus laevis Age: 0 Organ: oocyte Host: TOP10 Vector: pBluescript SK- Vector type: phagemid Insert digest: 5' EcoRI/XhoI 3' Stop Codon Status: without Description: cDNA from 2 ug polyA+ mRNA, oligo dT-primed using 5'-ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTT-3'. Library was mass excised (from lambda-ZAPII) using ExAssist phage. The resulting single-stranded phagemids were normalized by hybridization to biotinylated driver (prepared from the same library by PCR) to Cot11. After removal of hybrids and excess driver by streptavidin sepharose chromatography, the ss-phagemids were made double-stranded. Original library (X.laevis occytes, stages 5-6 mixed) constructed and donated by B. Blumberg (University of California, Irvine), normalization by J. Song.
