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cDNA library: Xenopus laevis oocyte

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Information for 1 library

oocyte

   1. Xenopus laevis oocyte

Xenopus laevis oocyte

Name: Xenopus laevis oocyte
Library ID: 1931
Organism: Xenopus laevis
Age: 0
Organ: oocyte
Host: TOP10
Vector: pBluescript SK-
Vector type: phagemid
Insert digest: 5' EcoRI/XhoI 3'
Stop Codon Status: without
Description: cDNA from 2 ug polyA+ mRNA, oligo dT-primed using 5'-ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTT-3'. Library was mass excised (from lambda-ZAPII) using ExAssist phage. The resulting single-stranded phagemids were normalized by hybridization to biotinylated driver (prepared from the same library by PCR) to Cot11. After removal of hybrids and excess driver by streptavidin sepharose chromatography, the ss-phagemids were made double-stranded. Original library (X.laevis occytes, stages 5-6 mixed) constructed and donated by B. Blumberg (University of California, Irvine), normalization by J. Song.

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