Biosciences Directorate logo

I.M.A.G.E. Consortium

“Sharing resources to achieve a common goal — the discovery of all genes”

blue/green horizontal line

cDNA library: Melton normalized mixed mouse pancreas 1 N1-MMS1

Home | Site Map | I.Q. search | Libraries by Species | Clones | Vectors | Primers | QC | Arrayed Clones by: Library | Tissue |

Information for 1 library


   1. Melton normalized mixed mouse pancreas 1 N1-MMS1

Melton normalized mixed mouse pancreas 1 N1-MMS1

Name: Melton normalized mixed mouse pancreas 1 N1-MMS1
Library ID: 1948
Organism: Mus musculus
Strain: ICR
Gender: both
Age: 0
Stage: mixed
Organ: pancreas
Tissue: pool of whole pancreas, pancreatic bud, Islets of Langerhans
Host: DH10B
Vector: pSPORT1
Vector type: phagemid
Insert digest: 5' SalI/NotI 3'
Stop Codon Status: without
Description: Five libraries representing EI0.5/12.5 pancreatic bud, E16.5 pancreas, newborn pancreas, adult pancreas, and adult islets of Langerhans were separately constructed using SuperScript Plasmid Library kit (Life Technologies). cDNA was made by oligo-dT priming using primer 5'-PGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3' and size-selected by column fractionation. Cloning was accomplished via 5' linkers: 5'-TCGACCCACGCGTCCG-3' and 3'-GGGTGCGCAGGC-5'. Libraries were amplified once on solid support and plasmid DNA from each library was prepared and mixed in equal amounts. The mixed library DNA was normalized by method #4 from Bonaldo, Lennon, and Soares (1996 Genome Research 6:791-806); 0.5 microgram single-stranded mixed library plasmid DNA was mixed with 5 micrograms PCR product representing mixed library inserts and hybridized to an Ecot of 6. Single-stranded (unhybridized) plasmids were isolated by hydroxyapatite chromatography and used to make this library. Library constructed and arrayed in the laboratory of D. Melton, Ph.D. (Harvard University).

horizontal blue line

Home | I.M.A.G.E. | ORFeome | CGAP | MGC | XGC | ZGC | CMLS | Lawrence Livermore | U Iowa | LLNL Disclaimer | HAIB Disclaimer
Web page maintained by
Email address:
Biological Questions and Comments to