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cDNA library: Fugu UT6 adult gut

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Information for 1 library


   1. Fugu UT6 adult gut

Fugu UT6 adult gut

Name: Fugu UT6 adult gut
Library ID: 1956
Organism: Fugu rubripes
Age: 0
Stage: adult
Organ: gut
Host: DH10B TonA
Vector: pBluescript-FL
Vector type: phagemid
Insert digest: 5' PflmI/PflmI 3'
Stop Codon Status: without
Description: pBluescript-FL is created from pBluescript II phagemid SK(+). Its BamHI-SmaI sites (in the MCS) were converted to BamHI-PflMI-SfiI-PflMI sites (SmaI is destroyed in the process). The rest of the vector is untouched. 1st strand cDNA was primed with an oligo(dT) primer. Double-stranded cDNA was ligated into Pf1MI adaptors and inserted into the two PflMI sites in the T3 (5') to T7 (3') direction. PflMi can be used to isolate the cDNA insert. Library materials provided by G. Elgar (UK MRC HGMP-RC) and constructed and donated by Drs. K. Kawakami, M. Sasaki, S. Sugano, K. Kikuchi, and S. Watabe (University of Tokyo, Institute of Medical Science and Laboratory of Aquatic Molecular Biology and Biotechnology). T3 should be used for sequencing the 5' end of clones and T7 for the 3' end.

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