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cDNA library: NIH_MGC_142

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Information for 1 library

brain/CNS

   1. NIH_MGC_142

NIH_MGC_142

Name: NIH_MGC_142
Library ID: 1959
Organism: Homo sapiens
Gender: both
Age: 0
Stage: embryo
Organ: brain/CNS
Tissue: normal brain, pooled
Host: DH10B TonA
Vector: pDNR-LIB
Vector type: plasmid
Insert digest: SfiI (directional)
Stop Codon Status: without
Description: Double-stranded cDNA was prepared from a pool of 40 cell line polyA+ RNAs (bladder - 2%, blood - 33.4%, brain - 5.6%, breast - 12.5%, colon - 4%, connective tissue - 1.4%, eye - 1%, intestine - 2.6%, kidnney - 2.2%, liver - 5.7%, lung - 10.8%, NK-cell - 5.2%, ovary - 4%, pharynx - 2.5%, prostate - 4.3%, salivary gland - 1.3%, and skin - 2.3%). 5' and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected to contain the >0.5 kb size fraction (other fractions present in NIH_MGC_141). Library created in the laboratory of M. Brownstein (NIMH, NIH).

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