Name: NIH_MGC_154 Library ID: 1977 Organism: Mus musculus Strain: C57BL/6 Gender: male Age: 0 Stage: adult Organ: kidney Tissue: DMSO control treated 48 hours IP injection, pool of 3 Host: DH10B TonA Vector: pDONR201 Vector type: plasmid Insert digest: 5' attP2/attP1 Stop Codon Status: without Description: cDNA made by oligo-dT with attB2 site and directionally cloned. Priming sequence: 5'-TTTCCTGCAGGCCGGCCACCACTTTGTACAAGAAAGCTGGGTTTTTTTTTTTTTTTTTTT-3'. Full-length enriched library was constructed using the GeneRacer kit by Invitrogen, library amplification 16 cycles. Library constucted by Mark Bittinger in the Bradfield laboratory (McArdle Laboratory for Cancer Research, University of Wisconsin). Note: this is a NIH_MGC Library.