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cDNA library: NIH_MGC_156

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Information for 1 library


   1. NIH_MGC_156


Name: NIH_MGC_156
Library ID: 1979
Organism: Mus musculus
Strain: C57BL/6
Gender: male
Age: 0
Stage: adult
Organ: heart
Tissue: Heart: TCDD (DMSO vehicle) treated 48 hours IP injection, pool of 3
Host: DH10B TonA
Vector: pDONR201
Vector type: plasmid
Insert digest: 5' attP2/attP1
Stop Codon Status: without
Description: cDNA made by oligo-dT with attB2 site and directionally cloned. Priming sequence: 5'-TTTCCTGCAGGCCGGCCACCACTTTGTACAAGAAAGCTGGGTTTTTTTTTTTTTTTTTTT-3'. Full-length enriched library was constructed using the GeneRacer kit by Invitrogen, library amplification 16 cycles. Library constucted by Mark Bittinger in the Bradfield laboratory (McArdle Laboratory for Cancer Research, University of Wisconsin). Note: this is a NIH_MGC Library.

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