Name: MAPcL Library ID: 1980 Organism: Homo sapiens Age: 0 Organ: mixed Tissue: Pooled cell lines Host: DH10B Vector: pCMV-SPORT6 Vector type: phagemid Insert digest: 5' EcoRV/NotI 3' Stop Codon Status: without Description: cDNA made by oligo-dT priming. Directionally cloned into EcoRV (site destroyed upon cloning) and NotI. The library was size-selected and went through 26K fold of amplification. Average insert size is 1.8 kb. Library constructed from cell lines ZR-75-1, MCF7, SK-BR-3, MDA-MB-231, hTERT-HME1, LNCaP and subtracted against brain, liver, lung, kidney and muscle. cDNA Library constructed by Life Technologies (Invitrogen). The ZR-75-1, MCF7, SK-BR-3, MDA-MB-231, and LNCaP cell lines were acquired from ATCC. The hTERT-HME1 cell line was purchased from Clontech. REFERENCE: Kristi A. Egland, James J. Vincent, Robert Strausberg, Bungkook Lee, and Ira Pastan: Discovery of new breast cancer genes encoding membrane and secreted proteins. Manuscript submitted. (National Cancer Institute, National Institutes of Health).