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cDNA library: MAPcL

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Information for 1 library

mixed

   1. MAPcL

MAPcL

Name: MAPcL
Library ID: 1980
Organism: Homo sapiens
Age: 0
Organ: mixed
Tissue: Pooled cell lines
Host: DH10B
Vector: pCMV-SPORT6
Vector type: phagemid
Insert digest: 5' EcoRV/NotI 3'
Stop Codon Status: without
Description: cDNA made by oligo-dT priming. Directionally cloned into EcoRV (site destroyed upon cloning) and NotI. The library was size-selected and went through 26K fold of amplification. Average insert size is 1.8 kb. Library constructed from cell lines ZR-75-1, MCF7, SK-BR-3, MDA-MB-231, hTERT-HME1, LNCaP and subtracted against brain, liver, lung, kidney and muscle. cDNA Library constructed by Life Technologies (Invitrogen). The ZR-75-1, MCF7, SK-BR-3, MDA-MB-231, and LNCaP cell lines were acquired from ATCC. The hTERT-HME1 cell line was purchased from Clontech. REFERENCE: Kristi A. Egland, James J. Vincent, Robert Strausberg, Bungkook Lee, and Ira Pastan: Discovery of new breast cancer genes encoding membrane and secreted proteins. Manuscript submitted. (National Cancer Institute, National Institutes of Health).

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