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cDNA library: NIH_MGC_135

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Information for 1 library


   1. NIH_MGC_135


Name: NIH_MGC_135
Library ID: 1996
Organism: Mus musculus
Age: 0
Stage: embryo
Organ: limb
Tissue: Embryonic limb, maxilla and mandible
Host: DH10B TonA
Vector: pCMV-SPORT6.1
Vector type: phagemid
Insert digest: 5' EcoRV/NotI 3'
Stop Codon Status: without
Description: Normalized full-length enriched library from pooled mouse embryonic limb, maxilla and mandible, day 12.5, 13.5, 14.5, and 15.5. Oligo-dT primed (5''- GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)16-3''), directionally cloned, size selected for the 0.5-1 kb fragments; average insert size 1.6 kb. Normalized to Cot 7.5. Tissue contributed by David Rowe. Library constructed by ResGen, Invitrogen Corp. Note: this is a NIH_MGC Library.

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